An 8-year old boy was admitted to a leading hospital in Vijaywada with on and off fever since 3 months, 2 episodes of seizures and shortness of breath since 2 weeks. A portion from the biopsy of the vegetation was sent for routine Microbial culture and a sample of the same was sent to iGenetic for the Infective Endocarditis panel. The microbial culture was negative while the extremely sensitive PCR method used at iGenetic was able to detect Staphylococcus aureus in the sample. iGenetic was also able to diagnose MRSA or Methicillin-Resistant Staphylococcus aureus. The patient was treated with an alternative antibiotic- Vancomycin in combination with Cefrtiaxone, and recovered.
A 2year old boy was admitted in the hospital with fever for 3 days, 2 episodes of left focal seizures on day 3 of fever, followed by encephalopathy. His CSF sample was sent to iGenetic for infection detection. The result was positive for HSV and CMV. A quick diagnosis allowed the clinician to target the treatment for the child and his fever subsided rapidly and sensorium improved
A 63-year old male patient at a leading Hyderabad hospital was admitted with shortness of breath since a month. The patient had a history of hypertension and diabetes mellitus and was on treatment for the same. The patient was also suffering from triple-vessel coronary artery disease with moderate mitral regurgitation. He had undergone coronary artery bypass graft surgery with mitral valve repair 5 weeks before current admission followed by a tracheostomy a week later. At admission, patient was found to be in sepsis and in need of urgent treatment. Using their rapid Molecular Diagnostic tests on the patient's wound swab, iGenetic Diagnostics was able identify the pathogen and its antibiotic resistance spectrum within 24 hours. Their Septiscreen panel detected Klebsiella pneumoniae and Acinetobacter baumannii, and their Antibiotic Resistance Molecular Detection Panel revealed that the bacteria were Extended Spectrum Beta Lactamase (ESBL) positive. The patient was treated with Meropenem and Polymixin-B but developed a drug reaction to the latter. So he was treated with Meropenem and Fosfomycin, and survived this episode of sepsis and was shifted to the ward.
A 73-year old female patient at a leading Cancer Hospital in Bengaluru suffering from Diffuse Large B-Cell Lymphoma (DLBCL) and receiving chemotherapy came in with fever since 4-5 days, weakness and decreased oral intake. The doctor suspected sepsis and started the patient with higher antibiotics. However, the patient responded poorly to treatment. The Doctor requested iGenetic to run the Septiscreen panel. iGenetic ran the required investigation directly on the patient's blood sample and reported the sample positive for Pneumocystis jiroveci (carinii) within 24 hours. Based on the report, the patient was started on the appropriate antibiotic immediately and showed remarkable improvement in 2 days and was discharged from the ICU.
An 82-year old male was admitted into a leading hospital in Indore six month after total knee replacement. He had complain of knee pain with swelling and had lost 20khs weight in 3 months. Multiple tests were performed including microbiology culture for pus sample that was negative. Additional oncological investigations were also performed which came out negative. CT-guided biopsy of an osteolytic area in the dorsal vertebra was also inconclusive.
Synovial fluid was sent to iGenetic for the Osteomyelitis Panel. Using this panel, we were able to detect Mycobacterium tuberculosis within 24 hours.
This case was challenging to diagnose and treat as the doctors did not suspect TB and did not test for the same. This is because TB infection in knee replacements is rare. However, with comprehensive coverage of a wide variety of pathogens in our Osteomyelitis Panel, iGenetic was able to diagnose a case that went undiagnosed for around 1.5 months - we unequivocally diagnosed it as TB within 24 hours.